Molecular weight (DA)
34kD
Immunogen
The antiserum was produced against synthesized peptide derived from human SIAH1. AA range:181-230
Specificity
Siah-1/2 Polyclonal Antibody detects endogenous levels of Siah-1/2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein that is a member of the seven in absentia homolog (SIAH) family. The protein is an E3 ligase and is involved in ubiquitination and proteasome-mediated degradation of specific proteins. The activity of this ubiquitin ligase has been implicated in the development of certain forms of Parkinson's disease, the regulation of the cellular response to hypoxia and induction of apoptosis. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008],
Function
domain:The RING-type zinc finger domain is essential for ubiquitin ligase activity.,domain:The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.,function:E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic signal
Protein name
E3 ubiquitin-protein ligase SIAH1/2
Other name
SIAH1; HUMSIAH; E3 ubiquitin-protein ligase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a; SIAH2; E3 ubiquitin-protein ligase SIAH2; Seven in absentia homolog 2; Siah-2; hSiah2
Fields
>>p53 signaling pathway;>>Ubiquitin mediated proteolysis;>>Wnt signaling pathway
Human protein sequence Database
Q8IUQ4/O43255
Mouse protein sequence database
Rat gene ID
140941/140593
Rat protein sequence database
Q920M9/Q8R4T2
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
Tissue expression
Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
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