Immunogen
Purified recombinant fragment of LysRS(aa90-174) expressed in E. Coli.
Specificity
LysRS Monoclonal Antibody detects endogenous levels of LysRS protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/200 - 1/1000. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
Affinity purification
Background
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,subunit:Homodimer (By similarity); also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.,
Protein name
Lysyl-tRNA synthetase
Other name
KARS; KIAA0070; Lysine--tRNA ligase; Lysyl-tRNA synthetase; LysRS
Fields
>>Aminoacyl-tRNA biosynthesis
Human protein sequence Database
Q15046
Mouse protein sequence database
Q99MN1
Rat protein sequence database
Cellular localization
[Isoform Cytoplasmic]: Cytoplasm, cytosol . Cytoplasm . Nucleus . Cell membrane ; Peripheral membrane protein . Secreted . Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739). .; [Isoform Mitochondrial]: Mitochondrion .
Tissue expression
Bone marrow,Brain,Cerebellum,Hepatoma,Placenta,
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