Molecular weight (DA)
100kD
Immunogen
The antiserum was produced against synthesized peptide derived from human N4BP1. AA range:383-432
Specificity
N4BP1 Polyclonal Antibody detects endogenous levels of N4BP1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
Function
PTM:Monoubiquitinated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the N4BP1 family.,subcellular location:In discrete circular structures.,subunit:Binds NEDD4.,tissue specificity:Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.,
Protein name
NEDD4-binding protein 1
Other name
N4BP1; KIAA0615; NEDD4-binding protein 1; N4BP1
Human protein sequence Database
O75113
Mouse protein sequence database
Q6A037
Rat protein sequence database
Cellular localization
Cytoplasm, cytosol . Nucleus . Nucleus, nucleolus . Nucleus, PML body . Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. .
Tissue expression
Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
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