Immunogen
Synthesized peptide of human CD15.
Specificity
CD15 Monoclonal Antibody detects endogenous levels of CD15 protein.
Formulation
Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Dilution rate
Immunohistochemistry: 1/200 - 1/1000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009],
Function
caution:It is uncertain whether Met-1 or Met-126 is the initiator.,function:May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.,online information:Fucosyltransferase 4,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 10 family.,subcellular location:Membrane-bound form in trans cisternae of Golgi.,
Protein name
Alpha-(1,3)-fucosyltransferase
Other name
FUT4; ELFT; FCT3A; Alpha-(1; 3)-fucosyltransferase; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
Fields
>>Mannose type O-glycan biosynthesis;>>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways
Human protein sequence Database
P22083
Mouse protein sequence database
Q11127
Rat protein sequence database
Cellular localization
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi.
Tissue expression
[Isoform Short]: Expressed at low levels in bone marrow-derived mesenchymal stem cells. ; Expressed in cord blood immature promyelocytes and in peripheral blood myeloid and lymphoid cell populations.
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