Molecular weight (DA)
22kD
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPL20. AA range:100-149
Specificity
MRP-L20 Polyclonal Antibody detects endogenous levels of MRP-L20 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/40000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 21q. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016],
Function
similarity:Belongs to the ribosomal protein L20P family.,
Protein name
39S ribosomal protein L20 mitochondrial
Other name
MRPL20; 39S ribosomal protein L20; mitochondrial; L20mt; MRP-L20
Human protein sequence Database
Q9BYag-9
Mouse protein sequence database
Q9CQL4
Rat protein sequence database
Cellular localization
Mitochondrion .
Tissue expression
Bone marrow,Lymph,Mammary gland,Whole embryo,