Molecular weight (DA)
42kD
Immunogen
Synthesized peptide derived from human Drosophila Melanogaster β-actin
Specificity
This antibody detects endogenous levels of Drosophila Melanogaster β-actin at Human, Mouse,Rat
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.13% sodium azide.
Dilution rate
IHC-p1:50-200 ,WB 1:1000-2000
Purification process (Immunogen)
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Background
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Gene Name
Drosophila Melanogaster β-actin
Protein name
Drosophila Melanogaster β-actin
Human protein sequence Database
P10987
Mouse protein sequence database
Rat protein sequence database
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