Molecular weight (DA)
192kD
Immunogen
Synthesized peptide derived from part region of human protein.AA1600-1700
Specificity
ZO1 Polyclonal Antibody detects endogenous levels of protein.
Formulation
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Dilution rate
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014],
Function
domain:The second PDZ domain mediates interaction with GJA12.,function:The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.,PTM:Phosphorylated.,similarity:Belongs to the MAGUK family.,similarity:Contains 1 guanylate kinase-like domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 SH3 domain.,similarity:Contains 1 ZU5 domain.,similarity:Contains 3 PDZ (DHR) domains.,subcellular location:Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact.,subunit:Interacts with HSPA4 and KIRREL1 (By similarity). Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with occludin, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1.,tissue specificit
Protein name
Tight junction protein ZO-1 (Tight junction protein 1) (Zona occludens protein 1) (Zonula occludens protein 1)
Fields
>>Adherens junction;>>Tight junction;>>Gap junction;>>Vibrio cholerae infection;>>Epithelial cell signaling in Helicobacter pylori infection;>>Pathogenic Escherichia coli infection
Human protein sequence Database
Q07157
Mouse protein sequence database
P39447
Rat protein sequence database
Cellular localization
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Cell junction . Cell junction, gap junction. Cell projection, podosome . Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225). .
Tissue expression
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车