Immunogen
Synthesized peptide derived from human CPSF5 AA range: 25-75
Specificity
This antibody detects endogenous levels of CPSF5 at Human/Mouse/Rat
Source
Polyclonal, Rabbit,IgG
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1：500-2000
Purification process (Immunogen)
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Background
The protein encoded by this gene is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides. [provided by RefSeq, Jul 2008],
Function
function:Component of the cleavage factor Im (CFIm) complex that plays a key role in pre-mRNA 3'-processing. Involved in association with CPSF6 in pre-MRNA 3'-end poly(A) site cleavage and poly(A) addition. NUDT21/CPSF5 binds to cleavage and polyadenylation RNA substrates. Binds to, but does not hydrolyze mono- and di-adenosine nucleotides. May have a role in mRNA export.,miscellaneous:Has no hydrolase activity as missing metal-binding sites in NUDIX motif.,PTM:Acetylated mainly by p300/CBP, recruited to the complex by CPSF6. Acetylation decreases interaction with PAPAO. Deacetylated by the class I/II HDACs, HDAC1, HDAC3 and HDAC10, and by the class III HDACs, SIRT1 AND SIRT2.,similarity:Belongs to the Nudix hydrolase family. CPSF5 subfamily.,similarity:Contains 1 nudix hydrolase domain.,subcellular location:In punctate subnuclear structures localized adjacent to nuclear speckles, called
Gene Name
NUDT21 CFIM25 CPSF25 CPSF5
Fields
>>mRNA surveillance pathway
Human protein sequence Database
O43809
Mouse protein sequence database
Q9CQF3
Rat protein sequence database
Q4KM65
Cellular localization
Nucleus . Cytoplasm . Shuttles between the nucleus and the cytoplasm in a transcription- and XPO1/CRM1-independent manner, most probably in complex with the cleavage factor Im complex (CFIm) (PubMed:19864460). In punctate subnuclear structures localized adjacent to nuclear speckles, called paraspeckles (PubMed:15169763). .
Tissue expression
Expressed in the heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
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