Molecular weight (DA)
41kD
Immunogen
The antiserum was produced against synthesized peptide derived from human CaMK1-alpha around the phosphorylation site of Thr177. AA range:143-192
Specificity
Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase. [provided by RefSeq, Jul 2008],
Function
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate.,enzyme regulation:Activated by Ca(2+)/calmodulin. Binding of calmodulin results in a conformational change that generates functional binding sites for both, substrate and ATP, and thus releaves intrasteric autoinhibition. Must be phosphorylated to be maximally active. Phosphorylated by CAMKK1 or CAMKK2.,function:Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes like transcriptional regulation, hormone production, translational regulation, regulation of actin filament organization and neurite outgrowth. Involved in calcium-dependent activation of the ERK pathway (By si
Protein name
Calcium/calmodulin-dependent protein kinase type 1
Other name
CAMK1; Calcium/calmodulin-dependent protein kinase type 1; CaM kinase I; CaM-KI; CaM kinase I alpha; CaMKI-alpha
Fields
>>Calcium signaling pathway;>>Oxytocin signaling pathway;>>Aldosterone synthesis and secretion;>>Glioma
Human protein sequence Database
Q14012
Mouse protein sequence database
Q91YS8
Rat protein sequence database
Q63450
Cellular localization
Cytoplasm . Nucleus . Predominantly cytoplasmic. .
Tissue expression
Widely expressed. Expressed in cells of the zona glomerulosa of the adrenal cortex.
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