Molecular weight (DA)
45kD
Immunogen
The antiserum was produced against synthesized peptide derived from human KAP2. AA range:41-90
Specificity
PKA IIα reg Polyclonal Antibody detects endogenous levels of PKA IIα reg protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
protein kinase cAMP-dependent type II regulatory subunit alpha(PRKAR2A) Homo sapiens cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has b
Function
function:Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.,PTM:Phosphorylated by the activated catalytic chain.,similarity:Belongs to the cAMP-dependent kinase regulatory chain family.,similarity:Contains 2 cyclic nucleotide-binding domains.,subunit:The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.,tissue specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.,
Protein name
cAMP-dependent protein kinase type II-alpha regulatory subunit
Other name
PRKAR2A; PKR2; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit
Fields
>>Insulin signaling pathway
Human protein sequence Database
P13861
Mouse protein sequence database
P12367
Rat protein sequence database
Cellular localization
Cytoplasm . Cell membrane . Colocalizes with PJA2 in the cytoplasm and the cell membrane.
Tissue expression
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
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