Immunogen
Purified recombinant human EF-2 protein fragments expressed in E.coli.
Specificity
EF-2 Monoclonal Antibody detects endogenous levels of EF-2 protein.
Formulation
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.
Dilution rate
Western Blot: 1/1000 - 1/2000. Not yet tested in other applications.
Purification process (Immunogen)
Affinity purification
Background
This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],
Function
function:This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome.,PTM:Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A.,PTM:Phosphorylation by EF-2 kinase completely inactivates EF-2.,similarity:Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.,subunit:Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9.,
Protein name
Elongation factor 2
Other name
EEF2; EF2; Elongation factor 2; EF-2
Fields
>>AMPK signaling pathway;>>Oxytocin signaling pathway
Human protein sequence Database
P13639
Mouse protein sequence database
P58252
Rat protein sequence database
P05197
Cellular localization
Cytoplasm . Nucleus . Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product. .
Tissue expression
Brain,Cajal-Retzius cell,Epithelium,Hepatocyte,Ovary,Periph
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