Molecular weight (DA)
173kD
Immunogen
The antiserum was produced against synthesized peptide derived from human ARHGEF12. AA range:449-498
Specificity
LARG Polyclonal Antibody detects endogenous levels of LARG protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB 1:500-2000;IHC-p 1:50-300
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014],
Function
disease:A chromosomal aberration involving ARHGEF12 may be a cause of acute leukemia. Translocation t(11;11)(q23;23) with MLL.,function:May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PDZ (DHR) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane upon stimulation.,subunit:Interacts with GNA12 and GNA13, probably through the RGS-like domain. Interacts with RHOA, PLXNB1 and PLXNB2. Interacts through its PDZ domain
Protein name
Rho guanine nucleotide exchange factor 12
Other name
ARHGEF12; KIAA0382; LARG; Rho guanine nucleotide exchange factor 12; Leukemia-associated RhoGEF
Fields
>>Vascular smooth muscle contraction;>>Axon guidance;>>Platelet activation;>>C-type lectin receptor signaling pathway;>>Regulation of actin cytoskeleton;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Tuberculosis;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer
Human protein sequence Database
Q9NZN5
Mouse protein sequence database
Q8R4H2
Rat protein sequence database
Cellular localization
Cytoplasm . Membrane . Translocated to the membrane upon stimulation. .
Tissue expression
Ubiquitously expressed. Isoform 2 is found in jejunum and testis.
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