Molecular weight (DA)
25kD
Immunogen
The antiserum was produced against synthesized peptide derived from human RAN. AA range:167-216
Specificity
Ran Polyclonal Antibody detects endogenous levels of Ran protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen re
Function
function:Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases.,function:GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle.,PTM:The N-terminus is blocked.,similarity:Belongs to the small GTPase superfamily. Ran family.,subcellular location:Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Monomer. Also forms a complex with CHC1 and interacts with the AR N-terminal poly-Gln region. The interaction with AR is inversely correlated with the poly-Gln length. Part of a complex consisting of RANBP9, Ran, DYRK1B and COPS5. Found in a nuclear export complex with RANBP3 and XPO1. Component of a nuclear
Protein name
GTP-binding nuclear protein Ran
Other name
RAN; ARA24; OK/SW-cl.81; GTP-binding nuclear protein Ran; Androgen receptor-associated protein 24; GTPase Ran; Ras-like protein TC4; Ras-related nuclear protein
Fields
>>Ribosome biogenesis in eukaryotes;>>Nucleocytoplasmic transport;>>Viral life cycle - HIV-1;>>Human T-cell leukemia virus 1 infection
Human protein sequence Database
P62826
Mouse protein sequence database
P62827
Rat protein sequence database
P62828
Cellular localization
Nucleus . Nucleus envelope . Cytoplasm, cytosol . Cytoplasm . Melanosome . Predominantly nuclear during interphase (PubMed:8421051, PubMed:12194828, PubMed:10679025). Becomes dispersed throughout the cytoplasm during mitosis (PubMed:8421051, PubMed:12194828). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). .
Tissue expression
Expressed in a variety of tissues.
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