Molecular weight (DA)
Molecular Weight
Calculated MW: 211 kDa; Observed MW: 130-140 kDa
Immunogen
A synthesized peptide derived from human Myosin light chain kinase
Dilution rate
WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 FC: 1/50-1/100
Purification process (Immunogen)
Background
Regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance.
Other name
KMLS; KRP; MLCK; MYLK; myosin light chain kinase; smooth muscle and non-muscle isozymes; myosin light polypeptide kinase; telokin
Human gene ID
SwissProt ID
Q15746
Human protein sequence Database
Gene ID
4638
Mouse protein sequence database
Rat protein sequence database
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.