Molecular weight (DA)
40kD
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246. AA range:207-256
Specificity
Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. ELISA: 1/10000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008],
Function
function:May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.,subcellular location:Found in the cytosolic fraction of the brain.,subunit:The phosphorylated form interacts with 14-3-3.,tissue specificity:Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).,
Protein name
Proline-rich AKT1 substrate 1
Other name
AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Fields
>>Autophagy - animal;>>mTOR signaling pathway;>>AMPK signaling pathway;>>Longevity regulating pathway;>>Longevity regulating pathway - multiple species;>>Thermogenesis;>>Shigellosis
Human protein sequence Database
Q96B36
Mouse protein sequence database
Q9D1F4
Rat protein sequence database
Cellular localization
Cytoplasm, cytosol . Found in the cytosolic fraction of the brain. .
Tissue expression
Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293).
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