Immunogen
The antiserum was produced against synthesized peptide derived from human HMG14. AA range:10-59
Specificity
HMG-14 Polyclonal Antibody detects endogenous levels of HMG-14 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMG17, the encoded protein may help maintain an open chromatin configuration around transcribable genes. [provided by RefSeq, Aug 2011],
Function
function:Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2.,mass spectrometry: PubMed:10739259,PTM:Phosphorylation on Ser-21 and Ser-25 weakens binding to nucleosomes and increases the rate of H3 phosphorylation (By similarity). Phosphorylation favors cytoplasmic localization.,RNA editing:Partially edited. A new initiator methionine may be created by a single uridine insertion in the 5'-UTR, causing an N-terminal extension of 45 amino acids. The existence of the RNA edited version is supported by direct protein sequencing by MS/MS of the following peptides specific to that version: 23-31 and 40-48. The RNA edited version is
Protein name
Non-histone chromosomal protein HMG-14
Other name
HMGN1; HMG14; Non-histone chromosomal protein HMG-14; High mobility group nucleosome-binding domain-containing protein 1
Human protein sequence Database
P05114
Mouse protein sequence database
P18608
Rat protein sequence database
Cellular localization
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus.
Tissue expression
Bone marrow,Brain,Epithelium,Liver,Ovary,Pooled,Skin,
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