Molecular weight (DA)
12kD
Immunogen
The antiserum was produced against synthesized peptide derived from mouse 4E-BP1 around the phosphorylation site of Thr69. AA range:35-84
Specificity
Phospho-4E-BP1 (T69) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T69.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Dilution rate
WB: 1/500 - 1/2000. IHC: 1/100 - 1/300. ELISA: 1/20000.. IF 1:50-200
Purification process (Immunogen)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Background
EIF4EBP1 encodes one member of a family of translation repressor proteins. The eukaryotic translation initiation factor 4E binding protein 1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Protein name
Eukaryotic translation initiation factor 4E-binding protein 1
Other name
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Human protein sequence Database
Mouse protein sequence database
Rat protein sequence database
Q62622
Contract Us
Follow Us
Location： 
Specification
成功添加到购物车